|
KMID : 0644120090220010041
|
|
Journal of Oriental Gynecology
2009 Volume.22 No. 1 p.41 ~ p.52
|
|
|
Effect of Prunellae Spica Extracts on Anti-tumor Metastasis by Immune Activity
|
|
Heo Ja-Kyung
Lee Jin-Moo
Lee Chang-Hoon
Cho Jung-Hoon
Jang Jun-Bock
Lee Kyung-Sub
|
|
|
Abstract
|
|
|
Purpose: This study was designed to investigate the anti-tumor metastasis by immunomodulating effects of extracts of Prunellae Spica.
Methods: Antimetastatic experiment was conducted in vivo by using colon 26-M3.1 carcinoma. And we observed cytotoxicity of Prunellae Spica on colon 26-M3.1 carcinoma, L5178Y-R lymphoma cell, hela cell and macrophage. To observe the immnomodulating effects of Prunellae Spica, we estimated IL-6, IL-10, IL-12, TNF- from peritoneal macrophages. And we evaluated the activation of NK cell by using anti-asialo-GM1 serum.
Results: We found that the administration of Prunellae Spica extracts significantly inhibited tumor metastasis in vivo. In an in vitro cytotoxicity analysis, cell growth are closer to 100% in case of colon 26-M3.1 carcinoma, L5178Y-R lymphoma cell, hela cell at low concentration. In case of macrophage, cell proliferation is closer to 100% less than of Prunellae Spica extracts. The level of cytokine such as IL-6, IL-10, IL-12 which stimulates Prunellae Spica extracts was increased in dose-dependent manner compared to the control group. TNF- is hardly secreted less than The depletion of NK cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Prunellae Spica on tumor metastasis.
Conclusion: Prunellae Spica appears to have considerable activity on the anti-metastasis by activation the immune system such as macrophage and NK cell.
|
|
|
KEYWORD
|
|
|
Prunellae Spica, anti-metastasis, immune system
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|